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Microsatellites for mahoganies: Twelve new loci for Swietenia macrophylla and its high transferability to Khaya senegalensis
Author(s) -
Lemes Maristerra R.,
Esashika Thana,
Gaoue Orou G.
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100074
Subject(s) - khaya , biology , swietenia macrophylla , meliaceae , microsatellite , population , botany , allele , genetics , demography , gene , sociology
• Premise of the study: A new set of 12 microsatellite markers was developed and characterized for big‐leaf mahogany ( Swietenia macrophylla King, Meliaceae) and its transferability assayed in the African mahogany, Khaya senegalensis (Desr.) A. Juss. (Meliaceae), to study population and conservation genetics of these threatened tropical timber species. • Methods and Results: Using an enriched library approach twelve novel microsatellite loci were identified for S. macrophylla . The number of alleles per locus ranged from 5 to 14 and mean observed and expected heterozygosities were 0.819 and 0.822, respectively. Twenty microsatellite loci developed for S. macrophylla (12 from this study and eight previously published) were tested for K. senegalensis and 10 polymorphic were characterized. • Conclusions: The results show the highly informative content of the new SSR loci for Swietenia macrophylla and the high effectiveness of these microsatellites for population genetics, gene flow, and mating system studies in Khaya senegalensis .