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Isolation and characterization of microsatellite loci from apomictic Hypericum perforatum (Hypericaceae)
Author(s) -
Corral Jose M.,
Molins Marta Puente,
Aliyu Olawale M.,
Sharbel Timothy F.
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100059
Subject(s) - biology , apomixis , microsatellite , polyploid , locus (genetics) , hypericum , loss of heterozygosity , hypericum perforatum , allele , genetics , botany , genome , ploidy , gene , pharmacology
• Premise of the study: Microsatellite markers were developed to study the origins and evolution of apomictic Hypericum perforatum , a polyploid invasive perennial herb indigenous to Europe that produces compounds of medicinal/pharmaceutical importance. • Methods and Results: Eleven primer sets were developed using a non‐radioactive protocol. All loci were polymorphic, showing from 2 to 9 alleles per locus. Observed and expected heterozygosity averaged 0.861 and 0.772, respectively, across four studied populations. Most primers also amplified successfully in eight other Hypericum species. • Conclusions: Markers developed in this study provide genetic tools for studies of apomixis and invasiveness, both on the intra‐ and interspecific levels.