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Development of polymorphic microsatellite markers for Incarvillea sinensis (Bignoniaceae)
Author(s) -
Yu HaiYan,
Luo YiBo,
Bai WeiNing
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100052
Subject(s) - biology , bignoniaceae , subcloning , microsatellite , locus (genetics) , botany , herb , allele , genetics , traditional medicine , medicinal herbs , gene , medicine , recombinant dna
• Premise of the study: Microsatellite markers were developed for Incarvillea sinensis var. sinensis (Bignoniaceae), an annual herb endemic to Inner Mongolia, to study the degree to which delayed self‐fertilization is favored. • Methods and Results: Eight polymorphic primer sets were isolated and characterized in two Inner Mongolia populations of I. sinensis var. sinensis with a relatively simple and fast subcloning method. Numbers of alleles per locus ranged from 2 to 7, with observed and expected heterozygosities ranging from 0 to 0.261 and from 0 to 0.778, respectively. • Conclusions: These markers will be useful for future studies of self‐fertilization adaptability in I. sinensis var. sinensis .
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