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Isolation of polymorphic microsatellite markers and tests of cross‐amplification in four widespread European calcicole ferns
Author(s) -
Groot G. Arjen,
Korpelainen Helena,
Wubs E. R. Jasper,
Erkens Roy
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100051
Subject(s) - biology , microsatellite , population , population genetics , evolutionary biology , genetics , allele , gene , demography , sociology
• Premise of the study: Studies on the biogeography and population genetics of the widespread European rock ferns Asplenium scolopendrium , A. trichomanes subsp. quadrivalens , Polystichum setiferum , and P. aculeatum would potentially yield interesting new insights into the colonization capacities of ferns. Markers with sufficient resolution for detailed genetic studies are, however, not yet available. • Methods and Results: Using genome screening with intersimple sequence repeat (ISSR) primers, a total of 16 different microsatellite markers were isolated and characterized for the four species. Some of these markers could be exchanged within each congeneric pair. • Conclusions: The developed primer sets will be very useful for analyses of the biogeography and population genetics of some widespread calcicole ferns. The observed cross‐amplification rates suggest a high potential for application on additional species from the same genera.