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Development and characterization of microsatellite markers for Panax notoginseng (Araliaceae), a Chinese traditional herb
Author(s) -
Liu Han,
Xia Tao,
Zuo YunJuan,
Chen ZhongJian,
Zhou ShiLiang
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100043
Subject(s) - panax notoginseng , microsatellite , biology , araliaceae , genetic diversity , locus (genetics) , loss of heterozygosity , herb , allele , traditional medicine , genetics , botany , medicinal herbs , gene , ginseng , population , medicine , alternative medicine , demography , pathology , sociology
• Premise of the study: Microsatellite primers were developed for a Chinese traditional herb, Panax notoginseng , to investigate its genetic diversity and cultivar breeding. • Methods and Results: Twelve polymorphic microsatellite loci were isolated from the microsatellite‐enriched genomic library of Panax notoginseng . The polymorphisms were assessed in two populations and an assemblage containing individuals from the entire distribution area. The number of alleles per locus ranged from 3 to 12, with a mean of 5.8; the observed and expected heterozygosity values ranged from 0.0411 to 0.8472 and from 0.0804 to 0.7653, respectively. • Conclusions: These new microsatellite markers will be useful for investigating the genetic diversity of this cultivated Panax notoginseng as well as assist in cultivar breeding.