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Isolation and characterization of microsatellite loci in Schoenoplectus juncoides (Cyperaceae) 1
Author(s) -
Ohsako Takanori,
Yamada Yuko
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100011
Subject(s) - biology , microsatellite , loss of heterozygosity , locus (genetics) , inbreeding , genetics , allele , microevolution , population , gene , demography , sociology
• Premise of the study : We developed microsatellite markers for Schoenoplectus juncoides , a weed found in rice paddy fields in eastern Asia. • Methods and Results : Ten dinucleotide microsatellite loci were isolated, and genetic variability within and among local populations were characterized. The number of alleles per locus ranged from 2 to 6 with a mean of 3.6. Expected heterozygosity per locus ranged from 0.067 to 0.471, whereas observed heterozygosity showed much lower values ranging from 0.000 to 0.083. Most of the loci showed significant deviation from Hardy−Weinberg equilibrium and high inbreeding coefficient values (0.525–1.000). • Conclusions : All the 10 microsatellite loci displayed polymorphism within and among populations. These loci are useful genetic markers for microevolution and ecological studies of S. juncoides populations.