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A set of novel microsatellite markers developed for the traditional Tibetan medicinal plant Halenia elliptica (Gentianaceae)
Author(s) -
Zhang ZhiRong,
Yang Jing,
Sun Yao,
Gao TianGang,
Li DeZhu,
Xue ChunYing
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000534
Subject(s) - gentianaceae , biology , microsatellite , gentiana , botany , traditional medicine , genetics , gene , allele , medicine
• Premise of the study: Microsatellite primers were developed in the traditional Tibetan medicinal plant Halenia elliptica D. Don to investigate its genetic diversity and population genetic structure. • Methods and Results: Using the Fast Isolation by AFLP of Sequences Containing (FIASCO) repeats protocol, 24 primer sets were identified in two wild populations. Of these primers, 12 displayed polymorphisms and 12 were monomorphic. The number of alleles per locus ranged from 2 to 6, with a mean of 3.9. The expected ( H E ) and observed ( H O ) heterozygosities ranged from 0.191 to 0.784 and from 0.417 to 0.917, respectively. All these primers successfully amplified in two close relatives of H. elliptica , Swertia bimaculata (Siebold & Zucc.) Hook. f. & Thomson ex C. B. Clarke and S. tetraptera Maxim. • Conclusions : These markers will facilitate further studies on the population genetics of Halenia elliptica and its allied species.