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Microsatellite markers in the traditional Chinese medicinal herb Gynostemma pentaphyllum (Cucurbitaceae)
Author(s) -
Liao Hui,
Zhao Yao,
Zhou Yin,
Wang Yuguo,
Wang Xiaofan,
Lu Fan,
Song Zhiping
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000456
Subject(s) - gynostemma pentaphyllum , biology , microsatellite , herb , population , genetic diversity , locus (genetics) , traditional medicine , allele , genetics , medicinal herbs , gene , medicine , chemistry , demography , chromatography , sociology , extraction (chemistry)
• Premise of the study: Microsatellite primers were developed for Gynostemma pentaphyllum , a traditional Chinese medicinal herb, to investigate its population genetic diversity. • Methods and Results: Using the Microsatellite Sequence Enrichment protocol, 14 polymorphic primers sets were identified in four Chinese Gynostemma pentaphyllum populations. The primers amplified di‐ and trinucleotide repeats with 1–6 alleles per locus, and the observed heterozygosities ranged from 0.000 to 1.000 per population. • Conclusions: These markers will facilitate further studies on genetic structure and gene flow of Gynostemma pentaphyllum populations.