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Genome‐specific SCAR markers help solve taxonomy issues: A case study with Sinapis arvensis (Brassiceae, Brassicaceae)
Author(s) -
Pankin Artem A.,
Khavkin Emil E.
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000422
Subject(s) - biology , sinapis , genome , brassicaceae , brassica , botany , genetics , taxonomy (biology) , hybrid , whole genome sequencing , evolutionary biology , gene
• Premise of the study: Traditional taxonomy and nomenclature of Brassiceae (Brassicaceae) species do not reflect their phylogeny. Revision of the species and generic limits supported by extensive molecular data seems crucial. • Methods and Results: Genome‐specific polymorphisms extracted from non‐coding and coding sequences were used to develop 14 s equence c haracterized a mplified r egion (SCAR) markers specific for the Brassica B genome. These SCARs were verified against 77 accessions of six U‐triangle Brassica species and used to screen 23 accessions of seven wild Brassiceae species to test for their cross‐species amplification. SCARs were found in all B‐genome Brassica species and also in Sinapis arvensis . • Conclusions: SCAR markers can be employed for discerning B‐genome chromosomes in Brassica species and S. arvensis to reliably identify B‐genome species and their natural hybrids. The combined molecular evidence supports the suggestion to revise the generic limits of Brassica and Sinapis .