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Development of microsatellite loci for Aconitum gymnandrum (Ranunculaceae), a species endemic to the Qinghai‐Tibetan Plateau
Author(s) -
Xu Tingting,
Chen Jian,
Zhu Lili,
Li Zhonghu
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000418
Subject(s) - biology , aconitum , microsatellite , locus (genetics) , ranunculaceae , plateau (mathematics) , population , genetic algorithm , genetic diversity , evolutionary biology , genetic structure , endemism , allele , genetic variation , ecology , genetics , botany , gene , mathematical analysis , alkaloid , mathematics , demography , sociology
• Premise of the study : Polymorphic microsatellite loci primers were developed in an alpine plant endemic to the Qinghai–Tibetan Plateau, Aconitum gymnandrum , to investigate its population genetic structure and cryptic speciation. • Methods and Results : Using the combined biotin capture method, 16 polymorphic microsatellite loci were identified in 66 individuals from six geographically distant populations of A. gymnandrum . Across all the A. gymnandrum samples, the number of alleles per locus ranged from 4 to 15. • Conclusions : These markers will be useful to study population structure and cryptic speciation of A. gymnandrum .