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Microsatellite markers for the Chinese herbaceous peony Paeonia lactiflora (Paeoniaceae)
Author(s) -
Li Lin,
Cheng Fangyun,
Zhang Qixiang
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000410
Subject(s) - paeonia lactiflora , microsatellite , biology , germplasm , herbaceous plant , genetic diversity , allele , genetics , botany , gene , medicine , population , alternative medicine , demography , pathology , sociology
• Premise of the study : Microsatellite (simple sequence repeat, SSR) primers were developed for the Chinese peony, P. lactiflora , to investigate the diversity within Chinese peony germplasm resources. • Methods and Results : Using an SSR‐enriched genomic library, a set of 10 unique polymorphic genomic SSRs was developed and characterized. The primers amplified 61 alleles in all 10 loci, including di‐, tri‐, and tetranucleotide repeats. The primers were also effective for P. veitchii and P. obovata . • Conclusions : The new primers will be useful for genetic research of the Chinese peony and extend the repertoire of SSR markers available to study the herbaceous taxa in Paeonia .