New primers for single‐copy nuclear‐encoded chloroplast‐expressed glutamine synthetase (ncpGS) in Oxalidaceae | Zendy

Oberlander Kenneth C. | Zendy; Dreyer Léanne L. | Zendy; Roets Francois | Zendy

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New primers for single‐copy nuclear‐encoded chloroplast‐expressed glutamine synthetase (ncpGS) in Oxalidaceae

Author(s) -

Oberlander Kenneth C.,

Dreyer Léanne L.,

Roets Francois

Publication year - 2010

Publication title -

american journal of botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.218

H-Index - 151

eISSN - 1537-2197

pISSN - 0002-9122

DOI - 10.3732/ajb.1000390

Subject(s) - biology , glutamine synthetase , chloroplast , genetics , evolutionary biology , glutamine , gene , amino acid

• Premise of the study: New primers were developed for the nuclear marker glutamine synthetase (ncpGS) in Oxalidaceae. • Methods and Results: New forward and reverse primers were designed and tested across a wide range of Oxalidaceae. Selected taxa were sequenced to confirm homology. Potential for phylogenetic study was assessed by comparing sequenced taxa with commonly used nuclear and plastid markers. • Conclusions: Four out of five Oxalidaceae genera and all tested Oxalis spp. amplified successfully. Sequencing confirmed homology of the amplicon. Parsimony analysis of ncpGS showed that it is a promising candidate for future phylogenetic work in Oxalidaceae.

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