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New primers for single‐copy nuclear‐encoded chloroplast‐expressed glutamine synthetase (ncpGS) in Oxalidaceae
Author(s) -
Oberlander Kenneth C.,
Dreyer Léanne L.,
Roets Francois
Publication year - 2010
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000390
Subject(s) - biology , glutamine synthetase , chloroplast , genetics , evolutionary biology , glutamine , gene , amino acid
• Premise of the study: New primers were developed for the nuclear marker glutamine synthetase (ncpGS) in Oxalidaceae. • Methods and Results: New forward and reverse primers were designed and tested across a wide range of Oxalidaceae. Selected taxa were sequenced to confirm homology. Potential for phylogenetic study was assessed by comparing sequenced taxa with commonly used nuclear and plastid markers. • Conclusions: Four out of five Oxalidaceae genera and all tested Oxalis spp. amplified successfully. Sequencing confirmed homology of the amplicon. Parsimony analysis of ncpGS showed that it is a promising candidate for future phylogenetic work in Oxalidaceae.