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Polymorphic microsatellite loci in Polemonium brandegei and P. viscosum (section Melliosoma , Polemoniaceae) 1
Author(s) -
Kulbaba Mason W.,
Worley Anne C.
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000365
Subject(s) - biology , microsatellite , locus (genetics) , allele , genetics , population , evolutionary biology , gene , demography , sociology
• Premise of the study : Microsatellite markers were isolated in Polemonium brandegei and P. viscosum to be used in future studies of mating system evolution, population structure, and hybridization. • Methods and Results : Six loci were used in a preliminary genetic diversity study in two populations each of the closely related Polemonium brandegei and P. viscosum . We found 39 alleles across the six loci (average 7 per locus), with overall levels of observed heterozygosities ranging from 0.067 to 0.867 in P. brandegei and 0.000 to 0.666 in P. viscosum . Additional primers are reported, but require further design and optimization. • Conclusions : The reported markers will aid in further studies of mating system evolution, population structure, and hybridization in P. brandegei and P. viscosum .