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Microsatellite markers in the Australian desert plant, Solanum centrale (Solanaceae)
Author(s) -
Waycott Michelle,
Jones Brendan L.,
Van Dijk Jent Kornelis,
Robson Heather L. A.,
Calladine Ainsley
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000356
Subject(s) - biology , microsatellite , solanum , genetic diversity , solanaceae , ploidy , allele , genetic marker , botany , genetics , population , gene , demography , sociology
• Premise of the study: The first microsatellite primers were developed for Solanum centrale , a native Australian species used in the bush foods industry. Existing markers developed for other Solanum species were also amplified. • Methods and Results: Using an enrichment cloning protocol, seven novel markers were developed, and 48 existing markers from other Solanum species were tested, resulting in the characterization of a set of six highly polymorphic co‐dominant loci for use in S. centrale . Microsatellite screening revealed polyploidy. Among the six highly polymorphic loci, allelic diversity ranged from 7 to 14. • Conclusions: These markers will be useful for investigating genetic diversity and as a simple way of estimating ploidy of wild populations.