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Isolation of compound microsatellite markers for the endangered plant Neolitsea sericea (Lauraceae)
Author(s) -
Zhai ShengNan,
Yan XiaoLing,
Nakamura Koh,
Mishima Misako,
Qiu YingXiong
Publication year - 2010
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000348
Subject(s) - biology , microsatellite , genetic diversity , lauraceae , endangered species , locus (genetics) , genetic structure , population , allele , genetic marker , genetics , evolutionary biology , zoology , botany , genetic variation , ecology , gene , demography , sociology , habitat
• Premise of the study: The development of compound microsatellite markers was conducted in Neolitsea sericea to investigate genetic diversity and population genetic structure of this endangered insular species. • Methods and Results: Using the compound microsatellite marker technique, 10 compound microsatellite markers that were successfully amplified showed polymorphism when assessed in 55 individuals from two populations in East China and Japan. Overall, the number of alleles ranged from 3 to 17, with an average of 7.9 alleles per locus. In addition, these primers could be easily amplified in Neolitsea aurata var. paraciculata and N. aurata var. chekiangensi s. • Conclusions: The highly polymorphic markers developed and characterized in this study will be useful for population genetic studies of N. sericea .