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Isolation and characterization of microsatellite loci in Cabralea canjerana (Meliaceae)
Author(s) -
Pereira Marlei F.,
Bandeira Ludmila F.,
Blanco Angel J. V.,
Coelho Alexandre S. G.,
Ciampi Ana Y.,
Franceschinelli Edivani V.
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000336
Subject(s) - biology , meliaceae , microsatellite , isolation (microbiology) , evolutionary biology , zoology , genetics , botany , allele , bioinformatics , gene
• Premise of the study: Microsatellite primers were developed in the native Neotropical tree species Cabralea canjerana (Vell.) Mart. (Meliaceae) to study population genetics in some Atlantic Forest fragments of Brazil. This species is suitable for use in reforestation and is commercially important due to its high‐quality wood. • Methods and Results: In this study, eight microsatellite loci were isolated from an enriched C. canjerana genomic library. These are the first microsatellite loci described for this genus. Genetic diversity analyses were carried out using 30 individuals and six polymorphic loci. An average of 11.2 alleles was found, and the observed heterozygosity was 0.65. • Conclusions: The microsatellite markers described here are valuable tools for further population genetic studies of the species.