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Development of microsatellite loci for the invasive weed Wedelia trilobata (Asteraceae)
Author(s) -
Wu Wei,
Zhou RenChao,
Huang HuiRun,
Ge XueJun
Publication year - 2010
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000327
Subject(s) - biology , microsatellite , amplified fragment length polymorphism , locus (genetics) , asteraceae , weed , population , botany , allele , genetics , genetic diversity , gene , demography , sociology
• Premise of the study : Microsatellite markers were developed to help elucidate the population genetics of the invasive species Wedelia trilobata . • Methods and Results : Using the Fast Isolation by AFLP of Sequences COntaining (FIASCO) repeats protocol, 23 sets of primers for amplifying microsatellite loci were identified in W. trilobata , 10 of which showed polymorphism (two to five alleles per locus) in samples of two populations of W. trilobata , one from China and one from Peru. Six of these loci were successfully amplified from samples of the native congener W. chinensis, with expected sizes. • Conclusions : These markers may be useful for further investigation of population genetics of Wedelia trilobata and other congener species.