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De novo sequencing and development of EST‐SSR for Gevuina avellana (Proteaceae), a nut tree from South America
Author(s) -
Riegel Ricardo,
Diaz Leila,
Véliz Débora
Publication year - 2010
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000314
Subject(s) - biology , proteaceae , microsatellite , genetic diversity , nut , expressed sequence tag , loss of heterozygosity , genetics , botany , allele , complementary dna , population , gene , demography , structural engineering , sociology , engineering
• Premise of the study: The aim of this study was to develop molecular tools to investigate the genetic structure and diversity of natural populations of Gevuina avellana (Molina, Proteaceae), the Chilean hazelnut. Simple sequence repeats (SSRs) derived from expressed sequence tags (EST‐SSRs) were developed, optimized, and characterized. • Methods and Results : The microsatellite‐containing sequences were selected from a cDNA library developed from the nut. The eight marker loci showed two to seven alleles in 60 unrelated trees, from two different natural populations. The observed heterozygosity (H o ) was 0.44, ranging from 0.07 to 0.92 for different loci. When the multilocus genotype of the eight EST‐SSRs was considered, all trees could be differentiated. • Conclusions: The newly identified EST‐SSRs in G. avellana and the multi‐pooling strategy involving eight markers will facilitate future studies of clone identification, genetic structure, and diversity.