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Development and characterization of microsatellite loci for Khaya senegalensis (Meliaceae) 1
Author(s) -
Sexton G. J.,
Frere C. H.,
Dieters M. J.,
Godwin I. D.,
Prentis P. J.
Publication year - 2010
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000300
Subject(s) - khaya , meliaceae , biology , microsatellite , evolutionary biology , botany , genetics , gene , allele
• Premise of the study : Microsatellite loci were developed to characterize genetic variation and population subdivision in Khaya senegalensis (Desr.) A. Juss. (Meliaceae). • Methods and Results : Microsatellite loci were identified from genomic DNA sequences generated using the 454 GS‐FLX titanium platform. Primers were designed for 67 tri‐ and tetranucleotide repeats, of which 20 were selected for 2 multiplexes based on amplification success and band size. Eleven of these loci showed polymorphism in two populations of Khaya senegalensis and amplified in individuals from across the species range. • Conclusions : These new microsatellite loci will be useful for investigation of the evolutionary and conservation genetics of Khaya senegalensis .