Development and characterization of microsatellite loci for  Khaya senegalensis  (Meliaceae) 1 | Zendy

Sexton G. J. | Zendy; Frere C. H. | Zendy; Dieters M. J. | Zendy; Godwin I. D. | Zendy; Prentis P. J. | Zendy

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Development and characterization of microsatellite loci for Khaya senegalensis (Meliaceae) 1

Author(s) -

Sexton G. J.,

Frere C. H.,

Dieters M. J.,

Godwin I. D.,

Prentis P. J.

Publication year - 2010

Publication title -

american journal of botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.218

H-Index - 151

eISSN - 1537-2197

pISSN - 0002-9122

DOI - 10.3732/ajb.1000300

Subject(s) - khaya , meliaceae , biology , microsatellite , evolutionary biology , botany , genetics , gene , allele

• Premise of the study : Microsatellite loci were developed to characterize genetic variation and population subdivision in Khaya senegalensis (Desr.) A. Juss. (Meliaceae). • Methods and Results : Microsatellite loci were identified from genomic DNA sequences generated using the 454 GS‐FLX titanium platform. Primers were designed for 67 tri‐ and tetranucleotide repeats, of which 20 were selected for 2 multiplexes based on amplification success and band size. Eleven of these loci showed polymorphism in two populations of Khaya senegalensis and amplified in individuals from across the species range. • Conclusions : These new microsatellite loci will be useful for investigation of the evolutionary and conservation genetics of Khaya senegalensis .

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