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Optimized genotyping with microsatellite markers in the fungal banana pathogen Mycosphaerella fijiensis (Mycosphaerellaceae)
Author(s) -
Robert Stéphanie,
Rieux Adrien,
Argout Xavier,
Carlier Jean,
Zapater MarieFrançoise
Publication year - 2010
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000295
Subject(s) - mycosphaerella , biology , genotyping , microsatellite , dna extraction , population , fungal pathogen , pathogen , genetics , botany , genotype , polymerase chain reaction , gene , demography , sociology , allele
• Premise of the study : Large‐scale population genetics studies are required to investigate the dispersal processes underlying the emergence of Mycosphaerella fijiensis , a fungal pathogen of banana. To this end, we have developed an optimized genotyping procedure combining novel microsatellite markers and a modified DNA extraction protocol. • Methods and Results : Primers for tetranucleotide loci were designed directly from the recently published genome sequence of M. fijiensis . A total of 19 new polymorphic and easy‐to‐score markers were developed. Their use was combined with an adapted protocol for total DNA extraction starting from young lesions collected from banana leaves, thus avoiding a pathogen isolation step. • Conclusions : The combination of the two technical developments presented here will permit the expansion of genotyping capacity in M. fijiensis , allowing large‐scale analysis of samples from various geographic locations.