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Development of new microsatellite markers from Mango ( Mangifera indica ) and cross‐species amplification
Author(s) -
Ravishankar Kundapura Venkataramana,
Mani Bellam HanumanthaReddy,
Anand Lalitha,
Dinesh Makki Ramachandra
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000263
Subject(s) - microsatellite , biology , mangifera , genetic diversity , locus (genetics) , allele , cultivar , genetic marker , genetics , botany , gene , population , demography , sociology
• Premise of the study: Microsatellite markers were developed and characterized to assess the genetic diversity among mango ( Mangifera indica ) cultivars and to test their amplification in closely related species. • Methods and Results: Thirty‐six microsatellite (simple sequence repeats; SSR) loci were isolated by a microsatellite‐enriched partial genomic library method. Primers designed for these loci were characterized using 30 diverse mango cultivars. The number of alleles ranged from 3 to 19 with an average of 9.2 alleles per locus. Polymorphic information content values ranged from 0.185 to 0.920 with a mean of 0.687. The total value for the probability of identity was 2.42 × 10 −31 . • Conclusions: The newly identified SSRs would be useful in genetic diversity studies, finger‐printing, and mapping. Loci from five related species, M . odorata , M. anadamanica , M. zeylanica , M. camptosperma , and M. griffithii, were successfully amplified using these SSR primers, showing their potential utility across species.