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Isolation and characterization of microsatellite markers in an endangered species Dracaena cambodiana (Liliaceae)
Author(s) -
Zhang Lu,
Li QiaoMing
Publication year - 2010
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000245
Subject(s) - microsatellite , biology , endangered species , amplified fragment length polymorphism , genetic diversity , population , genetics , ecology , allele , demography , sociology , habitat , gene
• Premise of the study: The development of microsatellite primers in the endangered species Dracaena cambodiana will be the foundation for genetic and conservation studies of D. cambodiana and several Dracaena species. • Methods and Results: A total of 26 microsatellite markers were developed in Chinese populations of D. cambodiana , using the Fast Isolation by AFLP of Sequences Containing Repeats (FIASCO) protocol. Among them, sixteen primer pairs generated polymorphic loci (fourteen of them successfully amplified in other four Dracaena species) and ten primer pairs produced monomorphic loci. • Conclusions: These microsatellite markers could be used in the further investigation of population genetics of D. cambodiana and other Dracaena species.