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Isolation and characterization of microsatellite loci in Santalum lanceolatum and Santalum leptocladum (Santalaceae)
Author(s) -
Jones Brendan L.,
Waycott Michelle,
Robson Heather L. A.,
Calladine Ainsley,
Page Tony
Publication year - 2010
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000213
Subject(s) - santalum album , biology , sandalwood , microsatellite , genetic diversity , evolutionary biology , genetics , botany , allele , gene , population , demography , archaeology , sociology , history
• Premise of the study: Microsatellite primers were developed for the first time in the native Australian sandalwood species Santalum lanceolatum . • Methods and Results: Using an enrichment cloning protocol, five novel polymorphic codominant loci were developed and characterized in S. lanceolatum and S. leptocladum . In addition to these, three existing microsatellite loci from other sandalwood species were successfully amplified and characterized for S. lanceolatum and S. leptocladum . Among the eight loci, allelic diversity ranged from 4 to 29. • Conclusions: Primers will be useful for studies of clonality, genetic diversity and spatial genetic structure in wild populations. When coupled with other molecular techniques will help investigate the relationship between S. lanceolatum and S. leptocladum , species of commercial and conservation interest.