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Development of microsatellite markers in Lupinus luteus (Fabaceae) and cross‐species amplification in other lupine species
Author(s) -
Gonzalez Lorena B. Parra,
Straub Shan C. K.,
Doyle Jeff J.,
Ortega Paula E. Mora,
Garrido Haroldo E. Salvo,
Butler Iván J. Maureira
Publication year - 2010
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000170
Subject(s) - biology , microsatellite , fabaceae , genetic diversity , locus (genetics) , lupinus , botany , allele , pyrosequencing , population , genetics , gene , demography , sociology
• Premise of the study : Microsatellite primers were developed in Lupinus luteus L., an emerging temperate protein crop, to investigate genetic diversity, population structure, and to facilitate the generation of better yellow lupine varieties. • Methods and Results: Thirteen polymorphic primer sets were evaluated in a European and Eastern European accession collection of L. luteus . The primers amplified di‐, tri‐, and tetranucleotide repeats with 2–4 alleles per locus. These revealed a moderate to low level of genetic variation, as indicated by an average observed heterozygosity of 0.0126. Select loci also amplified successfully in the closely related species L. hispanicus Boiss. & Reut. and in the New World species L. mutabilis Sweet. • Conclusions: These results indicate the utility of primers for the study of genetic diversity across L. luteus populations and related lupine species. The use of these microsatellite markers will facilitate the implementation of several molecular breeding strategies in yellow lupine.