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Generation and characterization of 11 novel est derived microsatellites from Vicia faba (Fabaceae)
Author(s) -
Gong YaMing,
Xu ShengChun,
Mao WeiHua,
Hu QiZan,
Zhang GuWen,
Ding Ju,
Li ZeYun
Publication year - 2010
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000166
Subject(s) - biology , vicia faba , microsatellite , loss of heterozygosity , expressed sequence tag , genetic diversity , fabaceae , sativum , pisum , allele , population , genetics , evolutionary biology , botany , gene , genome , demography , sociology
• Premise of the study: Simple sequence repeat (SSR) markers were developed for faba bean using expressed sequence tags (ESTs) from the NCBI database to study for genetic diversity. • Methods and Results: A total of 11 novel EST‐SSR loci were generated and characterized when tested on four populations of 29 faba bean individuals from China and Europe. The number of alleles (A) ranged from 1 to 3 in each population, and observed heterozygosity ( H O ) and expected heterozygosity ( H E ) ranged from 0 to 0.5000 and 0.6400, respectively. Furthermore, transferable analysis revealed that eight of these loci (72.73%) amplified in Pisum sativum L., six of which (75.00%) detected polymorphism. • Conclusions: The developed markers in this study will provide valuable tools for genetic diversity, resource conservation, genetic mapping, and marker‐assisted breeding of faba bean in the future.