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Development and characterization of polymorphic microsatellite markers in Momordica charantia (Cucurbitaceae)
Author(s) -
Wang ShuZhen,
Pan Lei,
Hu Kan,
Chen ChanYou,
Ding Yi
Publication year - 2010
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000153
Subject(s) - momordica , biology , cucurbitaceae , microsatellite , cucumis , population , locus (genetics) , subspecies , genetic diversity , allele , botany , lagenaria , cucurbita pepo , genetics , zoology , traditional medicine , gene , medicine , demography , sociology
• Premise of the study: Polymorphic microsatellite markers were developed for Momordica charantia L. to investigate the genetic diversity and population structure within and between M. charantia and its four related species ( Cucurbita pepo L., Luffa cylindrical L., Lagenaria siceraria L., and Cucumis sativus L.). • Methods and Results: Using the Fast Isolation by AFLP of Sequence COntaining Repeats (FIASCO) method, 16 polymorphic microsatellite loci were identified in 36 individuals of M. charantia . Across all the M. charantia samples, the number of alleles per locus ranged from three to eight. Seven primers successfully amplified in the four related species. • Conclusions: These markers will be useful to study population ecology and population differentiation among M. charantia species and its related species.