Premium
Development of a novel set of EST‐SSR markers and cross‐species amplification in Tamarix africana (Tamaricaceae)
Author(s) -
Terzoli Serena,
Beritognolo Isacco,
Sabatti Maurizio,
Kuzminsky Elena
Publication year - 2010
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000112
Subject(s) - biology , locus (genetics) , microsatellite , expressed sequence tag , loss of heterozygosity , allele , tamarix , genetic marker , genetics , genotype , evolutionary biology , botany , genome , gene
• Premise of the study: Tamarix plants are resistant to abiotic stresses and have become invasive in North America. Their taxonomy is troublesome, and few molecular makers are available to enable species identification or to track the spread of specific invasive genotypes. Transcriptome sequencing projects offer a potential source for the development of new markers. • Methods and Results: Thirteen polymorphic simple sequence repeats (SSRs) markers derived from Expressed Sequence Tags (ESTs) from Tamarix hispida, T. androssowii, T. ramosissima , and T. albiflonum were identified and screened on 24 samples of T. africana to detect polymorphism. The number of alleles per locus ranged from two to eight, with an average of 4.3 alleles per locus, and the mean expected heterozygosity was 0.453. • Conclusions: Amplification products of these 13 loci were also generated for T. gallica . These new EST‐SSR markers will be useful in genetic characterization of Tamarix , as additional tools for taxonomic clarification, and for studying invasive populations where they are a threat.