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Development of microsatellite loci for Pinus koraiensis (Pinaceae)
Author(s) -
Li Yong,
Liang Lu,
Ge XueJun
Publication year - 2010
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000098
Subject(s) - pinus koraiensis , biology , microsatellite , locus (genetics) , loss of heterozygosity , population , pinaceae , amplified fragment length polymorphism , genetics , allele , genetic diversity , evolutionary biology , gene , botany , pinus <genus> , demography , sociology
• Premise of the study: Microsatellite markers were developed for Pinus koraiensis to characterize its genetic diversity and understand its population structure. • Methods and Results: Using the Fast Isolation by AFLP of Sequences COntaining (FIASCO) Repeats protocol, 20 primer sets were developed in Chinese populations of P. koraiensis . Three of the markers showed polymorphism with two alleles per locus when assessed in a sample of two populations of P. koraiensis from the Changbai Mountain in the Jilin Province of China. Five and three loci were successfully amplified in P. taiwanensis and P. massoniana , respectively. The amplification size of these loci matches those in P. koraiensis . • Conclusions: These markers may be useful for further investigation of population genetics of P. koraiensis .
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