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Isolation and characterization of microsatellite loci in the common milkweed, Asclepias syriaca (Apocynaceae)
Author(s) -
Kabat Susan M.,
Dick Christopher W.,
Hunter Mark D.
Publication year - 2010
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000064
Subject(s) - biology , microsatellite , locus (genetics) , gene flow , genetics , population , allele , genetic marker , botany , gene , genetic variation , demography , sociology
• Premise of the study: Microsatellite primers were developed for the common milkweed, Asclepias syriaca L., to assist in genet identification and the analysis of spatial genetic structure. • Methods and Results: Using an enrichment cloning protocol, eight microsatellite loci were isolated and characterized in a Michigan population of A. syriaca . The primers amplified di‐ and trinucleotide repeats with 4–13 alleles per locus. • Conclusions: The primers will be useful for studies of clonality and gene flow in natural populations.