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Development of microsatellite markers for the moss Ptychomitrium gardneri (Ptychomitriaceae)
Author(s) -
Liu Yan,
Ge XueJun,
Sun QiBao,
Cao Tong
Publication year - 2010
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1000014
Subject(s) - biology , microsatellite , amplified fragment length polymorphism , disjunct , moss , genetic marker , locus (genetics) , population , genetics , allele , evolutionary biology , genetic diversity , botany , gene , demography , sociology
• Premise of the study: Microsatellite markers were developed for Ptychomitrium gardneri to study population genetics of this eastern Asian‐North American disjunct moss. • Methods and Results: A total of 13 microsatellite markers were developed in Chinese populations of P. gardneri , using the Fast Isolation by AFLP of Sequences COntaining Repeats protocol. Eight of the markers showed polymorphism when assessed in a sample of four populations of 29 individuals from China. These markers amplified three to four alleles per locus. Five primers also amplified in P. linearifolium and P. wilsonii . • Conclusions: These markers may be useful for further investigation of population genetics of P. gardneri .