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Microsatellite primers in Rhamnus cathartica (Rhamnaceae) and applicability in related taxa to assess hybridization events
Author(s) -
Culley Theresa M.,
Stewart J. Ryan
Publication year - 2010
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.0900394
Subject(s) - biology , microsatellite , locus (genetics) , primer (cosmetics) , taxon , rhamnaceae , genetics , allele , botany , gene , chemistry , organic chemistry
• Premise of the study: Microsatellite primers were developed in the introduced shrub Rhamnus cathartica L., to investigate potential hybridization events with related taxa as a stimulus of invasive spread. • Methods and Results: Using a non‐radioactive protocol, 15 primer sets were identified in North American and European populations of R. cathartica . The primers amplified di‐, tri‐ and pentanucelotide repeats with 1–14 alleles per locus. Most primers also amplified in R. lanceolata, R. smithii , and/or Frangula alnus (previously R. frangula ). • Conclusions: These results indicate the utility of primers in R. cathartica for future genetic studies as well as their potential applicability in other related taxa.