Open AccessCOLORIMETRIC VS. CHROMATOGRAPHIC ANALYSES OF ALKALOIDS IN LUPIN SEEDS
Author(s) -
Paulina Wilczura,
W. Święcicki,
Katarzyna Anna Kamel,
W. Wasiak
Publication year - 2018
Publication title -
plant breeding and seed science
Language(s) - English
Resource type - Journals
eISSN - 2083-599X
pISSN - 1429-3862
DOI - 10.37317/pbss-2018-0014
Subject(s) - lupinus , alkaloid , biology , cultivar , taste , gas chromatography , bitter taste , botany , horticulture , food science , chromatography , chemistry
A characteristic trait of lupins is a production of alkaloids, which are a toxic and bitter taste compound ofseeds. Due to the lack of fast, sensitive and inexpensive screening techniques to identify and reject high alkaloidplant material, development of suitable tools is important challenges for lupins breeding and seed production.The aim of this study was to compare two alkaloid content estimation methods in Lupinus angustifoliusL. and Lupinus albus L.During the Wagner’s colorimetric test, which is recommended by the UPOV, seed halves were stained onfour colors depending on the alkaloid content but only the level of 0.5% – 0.6% showed clear color change.Gas chromatography allowed accurate quantification and qualification of alkaloid content.Since safe alkaloid content for consumption is 0.02% of seed dry weight, colorimetric method is less usefulfor dividing lupin cultivars into sweet and bitter, than gas chromatography but can be used as a screeningtechnique.