Open AccessEnzyme Linked Immunosorbent Assay (ELISA)
Author(s) -
Maya Chandra Dita
Publication year - 2021
Publication title -
natural sciences engineering and technology journal
Language(s) - English
Resource type - Journals
ISSN - 2807-2820
DOI - 10.37275/nasetjournal.v1i2.6
Subject(s) - immunoassay , chromogenic , conjugate , antigen , enzyme , antibody , chemistry , chromatography , covalent bond , reagent , biochemistry , microbiology and biotechnology , biology , immunology , organic chemistry , mathematical analysis , mathematics
Enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA) are both widely used as diagnostic tools in medicine and as quality control measures in many industries. ELISA has been the system of choice when testing soluble antigens and antibodies. EIA / ELISA uses the basic immunological concept of antigen binding to specific antibodies, which allows the detection of small amounts of antigens such as proteins, peptides, hormones or antibodies in fluid samples. In all protocols, solid-phase reagents are incubated with secondary or tertiary reactants covalently coupled with the enzyme. The unbound conjugate is washed and a chromogenic or fluorogenic substrate is added.