Open AccessMicroRNA-152 Inhibits Cell Proliferation, Migration, and Invasion in Breast Cancer
Author(s) -
Adilijiang Maimaitiming,
Ailijiang Wusiman,
Abulajiang Aimudula,
Xuekelaiti Kuerban,
Pengcheng Su
Publication year - 2020
Publication title -
oncology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 57
eISSN - 1555-3906
pISSN - 0965-0407
DOI - 10.3727/096504019x15519249902838
Subject(s) - breast cancer , cancer research , microrna , metastasis , cell growth , rock1 , cancer , biology , cell migration , ca15 3 , ca 15 3 , cancer cell , medicine , cell , signal transduction , gene , microbiology and biotechnology , genetics , rhoa , biochemistry
The aim of the present study was to investigate the roles of microRNA-152 (miR-152) in the initiation and progression of breast cancer. The expression level of miR-152 was detected in human breast cancer tissue and a panel of human breast cancer cell lines using qRT-PCR. Results found that miR-152 expression was significantly downregulated in breast cancer tissue samples compared to adjacent noncancerous tissues as well as in breast cancer cell lines. Overexpression of miR-152 significantly suppressed breast cancer cell proliferation, migration, and invasion. Luciferase reporter assay results found that ROCK1 is a direct and functional target gene of miR-152 in breast cancer. In addition, downexpression of ROCK1 could inhibit breast cancer cell proliferation, migration, and invasion. These findings indicate that miR-152 inhibited breast cancer growth and metastasis through negative regulation of ROCK1 expression. These data suggest that miR-152/ROCK1 pathway may be a useful therapeutic target for breast cancer treatment.