Open AccessPlasmid-Based Stat3 siRNA Delivered by Functional Graphene Oxide Suppresses Mouse Malignant Melanoma Cell Growth
Author(s) -
Di Yin,
Yang Li,
Baofeng Guo,
Zhewen Liu,
Yang Xu,
Xiaoqin Wang,
Yanwei Du,
Libo Xu,
Yan Meng,
Xin Zhao,
Ling Zhang
Publication year - 2016
Publication title -
oncology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 57
eISSN - 1555-3906
pISSN - 0965-0407
DOI - 10.3727/096504016x14550280421449
Subject(s) - rna interference , transfection , polyethylenimine , small interfering rna , plasmid , gene delivery , genetic enhancement , cancer research , microbiology and biotechnology , stat3 , peg ratio , cell growth , chemistry , biology , gene , rna , biochemistry , finance , economics
RNA interference (RNAi) has been used for cancer gene therapy in recent years. However, the application of RNAi is hindered in the absence of safe and efficient gene delivery. In this article, a novel vehicle of graphene oxide functionalized with polyethylenimine and polyethylene glycol (GO-PEI-PEG) was successfully synthetized and then used to deliver plasmid-based Stat3 siRNA. The carrier can readily bind plasmid with high transfection efficiency. Moreover, molecular biology studies reveal that Stat3-related gene and protein expressions were significantly inhibited, suggesting that the formation of GO-PEI-PEG complexes could be utilized as a promising gene delivery in cancer therapy.