Open AccessInhibition of Migration and Invasion by Tet-1 Overexpression in Human Lung Carcinoma H460 Cells
Author(s) -
Si Jun Park,
Bo Ram Lee,
Hyeng-Soo Kim,
Young Rae Ji,
Yong Hun Sung,
Kwang ShikChoi,
Hum Dai Park,
Sung-Hyun Kim,
Myoung Ok Kim,
Zae Young Ryoo
Publication year - 2016
Publication title -
oncology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 57
eISSN - 1555-3906
pISSN - 0965-0407
DOI - 10.3727/096504015x14496932933539
Subject(s) - vimentin , metastasis , epithelial–mesenchymal transition , cancer research , lung cancer , cell culture , cadherin , biology , pathology , cell migration , cancer , carcinoma , cell , cancer cell , chemistry , medicine , immunohistochemistry , genetics
In the present study, we found that lung cancer cell line (H460 cells) expressing Tet1 showed higher levels of adhesion, and Tet1 inhibited H460 cell proliferation. In addition, these cells showed a significantly reduced ability of collagen degradation and Smad2/3 phosphorylation compared to controls. Furthermore, vimentin was found to be highly expressed in larger metastatic cancer area. Tet1 overexpression was reduced in the epithelial marker E-cadherin. Moreover, Tet1 repressed cancer cell metastasis in nude mice. Collectively, these findings suggest that Tet1 expression plays a critical role in metastasis of lung cancer cells by suppression of invasion and epithelial-mesenchymal transition (EMT).