Open AccessSynergistic Antitumoral Effect of IL-12 Gene Cotransfected With Antiangiogenic Genes for Angiostatin, Endostatin, and Saxatilin
Author(s) -
Hong Sung Kim,
Hyun Chul Jeong,
Yeon Kyung Lee,
Keun Sik Kim,
Yong Serk Park
Publication year - 2014
Publication title -
oncology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 57
eISSN - 1555-3906
pISSN - 0965-0407
DOI - 10.3727/096504014x13907540404798
Subject(s) - angiostatin , endostatin , cationic liposome , angiogenesis , cancer research , genetic enhancement , immune system , microbiology and biotechnology , biology , transfection , gene , chemistry , immunology , pharmacology , biochemistry
Previously, it was reported that the cotransfection of angiostatin K1-3, endostatin, and saxatilin genes using cationic liposomes significantly inhibited tumor progression. IL-12 is a well-known immune modulator that promotes Th1-type antitumor immune responses and also induces antiangiogenic effects. In this study, we have examined the antitumoral function of the IL-12 gene cotransfected with antiangiogenic genes for angiostatin K1-3, endostatin, and saxatilin by O,O ′-dimyristyl- N -lysyl glutamate (DMKE) cationic liposomes in a mouse tumor model. According to our results, the administration of the IL-12 gene or the genes for angiostatin K1-3, endostatin, and saxatilin exhibited effective inhibition of B16BL6 melanoma growth in mice. In particular, intravenous administration of the IL-12 gene along with intratumoral administration of the three antiangiogenic genes synergistically inhibited the B16BL6 tumor growth. These results suggest that systemically expressed IL-12 enhances antitumoral efficacy of locally expressed antiangiogenic proteins.