Open AccessAnalysis of Inter-(Simple Sequence Repeat) PCR Products From Human Colorectal Cancers
Author(s) -
Neng Chen,
Daniel L. Stoler,
Smitha S. Dutt,
G P Jahreis,
Miguel A. Rodrı́guez-Bigas,
Nicholas J. Petrelli,
Garth R. Anderson
Publication year - 2005
Publication title -
oncology research
Language(s) - English
Resource type - Journals
eISSN - 1555-3906
pISSN - 0965-0407
DOI - 10.3727/096504005776382297
Subject(s) - biology , genetics , genome instability , genome , polymerase chain reaction , sequence (biology) , computational biology , chromosome , gene , dna , dna damage
Genomic instability is a fundamental characteristic of solid tumors, and understanding genomic instability should significantly clarify the process of tumorigenesis. We adapted the sampling technique of inter-(simple sequence repeat) PCR [inter-(SSR) PCR] to measure genetic alterations between simple sequence repeats in colorectal tumors. It becomes important to precisely define both normal and altered inter-(SSR) PCR products. BLAT searches of 131 cloned inter-(SSR) PCR sequences reveal that inter-(SSR) PCR products are located on almost all the chromosomes except chromosome Y, indicating that inter-(SSR) PCR samples a representative diverse range of the genome. We confirm that a change in the pattern of the inter-(SSR) PCR products as seen on gel electrophoresis reflects a true alteration within the genome.