Open AccessMolecular cloning, sequence analysis and stress-related changes of the heat shock protein 60 gene in <I>Neobenedenia melleni</I>
Author(s) -
Fang Wang,
Jiong Chen,
Yu-Hong Shi,
Xin-Jiang Lu,
Mingyun Li
Publication year - 2013
Publication title -
dongwuxue yanjiu
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 19
ISSN - 0254-5853
DOI - 10.3724/sp.j.1141.2012.06603
Subject(s) - degree celsius , salinity , heat shock protein , complementary dna , biology , cloning (programming) , chaperone (clinical) , hsp60 , ectotherm , western blot , hsp90 , gene , temperature salinity diagrams , gene expression , hsp70 , microbiology and biotechnology , genetics , ecology , medicine , thermodynamics , physics , pathology , computer science , programming language
Heat shock protein 60 is an essential chaperone that can maintain the natural structure and function of mitochondrial proteins. Here, we successfully cloned the full length cDNA of HSP60 from Neobenedenia melleni, designated as NmHSP60. Real-time quantitative PCR and Western blot were used to analyze the expression change of NmHSP60 under different temperature and salinity. Compared with the typical 25 Degrees Celsius, expressions decreased dramatically in eggs and adults at 18 Degrees Celsius. Conversely, at 32 Degrees Celsius, expression increase dramatically in adults. Compared with salinity 24, expressions were significantly down-regulated in adults at salinity 18, and up-regulated in eggs at salinity 30. Experimental results suggest that NmHSP60 may play an significant role in N. melleni's adaptation to adverse environmental conditions.