Open AccessSimultaneous determination of 14 restricted substances in flavor and fragrant by ultra high performance liquid chromatography
Author(s) -
Jing Li,
Jing Xu,
Xuemei Li,
Jianguang Zhou,
Yan Zhu,
Miao Ming-ming
Publication year - 2013
Publication title -
sepu/chinese journal of chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.171
H-Index - 19
eISSN - 1872-2059
pISSN - 1000-8713
DOI - 10.3724/sp.j.1123.2012.03029
Subject(s) - chromatography , chemistry , detection limit , ammonium acetate , analyte , acetic acid , acetonitrile , reproducibility , aqueous solution , chromatography detector , flavor , elution , high performance liquid chromatography , analytical chemistry (journal) , organic chemistry , biochemistry
An ultra high performance liquid chromatographic method was established for the separation and determination of 14 restricted substances in flavor and fragrant samples. The sample was extracted by 10% (v/v) methanol aqueous solution containing 1% (v/v) ammonia. The 14 analytes were separated in 12 min on a Waters BEH C18 column (50 mm x 2.1 mm, 1.7 microm) using acetonitrile and 10 mmol/L ammonium acetate aqueous solution (containing 0.1% (v/v) acetic acid) as mobile phases with gradient elution at a flow rate of 0.2 mL/min and 35 degrees C, and detected by a diode array detector scanned from 200 nm to 500 nm. The regression equations revealed acceptable linearity (correlation coefficients ranged from 0.995 0 to 0.999 9) in the range of 0.10 - 50 mg/L for the 14 analytes. The limits of detection (LODs) were from 0.32 mg/kg to 2.51 mg/kg. The recoveries of the 14 analytes spiked in real samples at 5, 10 and 20 mg/L were 93.0% - 121% with the relative standard deviations (RSDs) of 0.51% - 4.50%. With the advantages of accessibility, high sensitivity and good reproducibility, this simple method can be used in the simultaneous determination of the restricted substances in flavor and fragrant samples.