Open AccessDevelopment of two-dimensional micro strong cation exchange liquid chromatography/reversed phase pressurized capillary electrochromatography and its application on <I>Cortex Phellodendri</I> extract
Author(s) -
Wu Yi,
Wei Yan,
Xue Gu,
Lin Zhang,
Yan Chen
Publication year - 2010
Publication title -
sepu/chinese journal of chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.171
H-Index - 19
eISSN - 1872-2059
pISSN - 1000-8713
DOI - 10.3724/sp.j.1123.2010.00226
Subject(s) - capillary electrochromatography , chromatography , chemistry , electrochromatography , resolution (logic) , capillary electrophoresis , high performance liquid chromatography , capillary action , elution , phase (matter) , analytical chemistry (journal) , materials science , organic chemistry , artificial intelligence , computer science , composite material
Pressurized capillary electrochromatography (pCEC) combines capillary electrophoresis (CE) with high performance liquid chromatography (HPLC), resulting in high separation efficiency, high selectivity, high resolution and fast speed. In addition, it provides a solvent gradient capability. Based on pCEC, a two-dimensional chromatography was constructed using micro strong cation exchange liquid chromatography and reversed phase pressurized capillary electrochromatography (micro-SCXLC/RP-pCEC) for the analysis of Cortex Phellodendri extract. A sample was separated by the first dimensional SCX column with a linear salt gradient elution into 11 plugs. Each plug was collected and injected into the second dimensional RP-pCEC column with a linear gradient for further separation. Compared with one-dimensional liquid chromatography, it has higher resolution and larger peak capacity. The two-dimensional chromatographic system is suitable for the separation and analysis of complex samples.