Open AccessRecent advances in the techniques of protein-protein interaction study
Author(s) -
Mingqiang Wang,
Jinxia Wu,
Yuhong Zhang,
Ning Han,
Hongwu Bian,
Zhu Muyuan
Publication year - 2013
Publication title -
yichuan
Language(s) - English
Resource type - Journals
ISSN - 0253-9772
DOI - 10.3724/sp.j.1005.2013.01274
Subject(s) - bimolecular fluorescence complementation , förster resonance energy transfer , surface plasmon resonance , protein–protein interaction , tandem , energy transfer , nanotechnology , immunoprecipitation , abiotic component , chemistry , yeast , biophysics , computational biology , fluorescence , biochemical engineering , computer science , biology , materials science , biochemistry , physics , chemical physics , ecology , engineering , gene , quantum mechanics , nanoparticle , composite material
Protein-protein interactions play key roles in the development of organisms and the response to biotic and abiotic stresses. Several wet-lab methods have been developed to study this challenging area,including yeast two-hybrid system, tandem affinity purification, Co-immunoprecipitation, GST Pull-down, bimolecular fluorescence complementation, fluorescence resonance energy transfer and surface plasmon resonance analysis. In this review, we discuss theoretical principles and relative advantages and disvantages of these techniques,with an emphasis on recent advances to compensate for limitations.