Open AccessA brief protocol for high-resolution whole mount in situ hybridization in zebrafish
Author(s) -
Chun Xia Zhang,
Feng Liu
Publication year - 2013
Publication title -
yichuan
Language(s) - English
Resource type - Journals
ISSN - 0253-9772
DOI - 10.3724/sp.j.1005.2013.00522
Subject(s) - zebrafish , troubleshooting , in situ hybridization , mount , computer science , fish <actinopterygii> , protocol (science) , biology , computational biology , gene expression , gene , genetics , fishery , operating system , medicine , alternative medicine , pathology
Whole mount in situ hybridization (WISH) is a popular technique that is used to study temporal-spatial gene expression at mRNA level in model systems. Sensitive and high-resolution WISH is important for every laboratory that uses this technique to study expression pattern of genes in wildtype and perturbed embryos. Based on the commonly used protocols in fish community, our lab optimizes them to get more sensitive and specific results. Here, we briefly summarize the history of this technology using zebrafish as an example, describe a detailed experimental protocol, and provide troubleshooting for imperfect results and their solution.