Open AccessAssociation of single nucleotide polymorphism of <I>RB1</I> gene with body weight traits in chicken
Author(s) -
Xi Chen,
Hui Zhang,
Yuxiang Wang,
Shouzhi Wang,
Baoping Cheng,
Hui Li
Publication year - 2012
Publication title -
yichuan
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.125
H-Index - 16
ISSN - 0253-9772
DOI - 10.3724/sp.j.1005.2012.01320
Subject(s) - single nucleotide polymorphism , haplotype , genetics , biology , genotype , polymorphism (computer science) , broiler , candidate gene , genetic association , gene , zoology
The Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) were used in the current study to investigate the effects of Retinoblastoma1 (RB1) gene on chicken body weight (BW). The single nucleotide polymorphisms (SNPs) of the individuals were detected by MALDI-TOF-MS and PCR-RFLP methods and the genotypes of 27 SNPs were obtained. Haplotypes were constructed by liding window approach. Association analysis between single SNPs and haplotypes and body weight were conducted, respectively. Based on the results of single SNPs and haplotype association analyses. Four SNPs with significant effects on body weight at 1 week of age (BW1) and 2 SNPs with significant effects on BW1 and BW3 were determined. These results suggested that RB1 is an important candidate gene that affects chicken early growth and development.