Open AccessProgression on genetic knockout tools in <I>Mycobacterium</I>
Author(s) -
Qin-Lin Du,
Xiangyu Fan,
Jinxiao Mao,
Jianping Xie
Publication year - 2012
Publication title -
yichuan
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.125
H-Index - 16
ISSN - 0253-9772
DOI - 10.3724/sp.j.1005.2012.00857
Subject(s) - obstacle , mycobacterium , biology , computational biology , mycobacterium avium complex , knockout mouse , gene knockout , genetics , microbiology and biotechnology , gene , political science , bacteria , law
Pathogenic mycobacteria were and remain a heavy burden to public health. Unfortunately, genetic manipulation including knockout technologies of Mycobacterium is difficult compared with other traditional model organisms. To overcome this obstacle, achievements in Mycobacterium knockout technologies were summarized, including delivery vector, sequence-specific recombination system, as well as the recently developed recombinogenic engineering and its application. The future for this tool innovation is also addressed.