Open AccessEffect of TSA and VPA treatment on long-tailed macaque (<I>Macaca fascicularis</I>)-pig interspecies somatic cell nuclear transfer
Author(s) -
Qin ZuXing,
Gao-Bo Huang,
Jun Luo,
Shu-Fang Ning,
Sen Lu,
LU Ke-huan
Publication year - 2012
Publication title -
yichuan
Language(s) - English
Resource type - Journals
ISSN - 0253-9772
DOI - 10.3724/sp.j.1005.2012.00342
Subject(s) - somatic cell nuclear transfer , blastocyst , trichostatin a , embryo , somatic cell , biology , embryonic stem cell , histone deacetylase inhibitor , macaque , in vitro , andrology , histone deacetylase , microbiology and biotechnology , embryogenesis , genetics , histone , neuroscience , medicine , gene
Long-tailed macaque-pig interspecies somatic cell nuclear transfer (iSCNT) is beneficial to yield embryonic stem cells from iSCNT embryos with similar genetic background as human, which can be used as materials for medical and basic research. The primary objective of this study was to investigate the effects of concentrations and treatment duration of two histone deacetylase inhibitors-Trichostatin A (TSA) and Valproic acid (VPA) and two different embryo culture media (PZM-3 and HECM-10) on the in vitro development of iSCNT embryos. The results suggested that when PZM-3 was used as the embryo culture medium, the blastocyst rate of 10 nmol/L TSA treatment for 48 h was significantly higher than the control group (22.78% vs 9.86%, P< 0.05). However, neither in PZM-3 nor in HECM-10, 2-10 mmol/L VPA treatment did not increase the in vitro developmental potential of iSCNT embryos. It was concluded that TSA treatment could enhance the in vitro developmental potential of long-tailed macaque-pig iSCNT embryos.