T-DNA integration patterns in transgenic plants mediated by Agrobacterium tumefaciens | Zendy

Lin Yang | Zendy; Fengling Fu | Zendy; Wan-Chen Li | Zendy

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T-DNA integration patterns in transgenic plants mediated by <I>Agrobacterium tumefaciens</I>

Author(s) -

Lin Yang,

Fengling Fu,

Wan-Chen Li

Publication year - 2011

Publication title -

hereditas (beijing)

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.125

H-Index - 16

ISSN - 0253-9772

DOI - 10.3724/sp.j.1005.2011.01327

Subject(s) - agrobacterium tumefaciens , transformation (genetics) , biology , agrobacterium , transgene , genetics , transfer dna , genetically modified crops , dna , gene

The genetic transformation mediated by Agrobacterium tumefaciens has been widely applied to research of transgenic plants. As the vector of the exotic genes, the integration patterns of T-DNA fragments affects not only transformation efficiency and stability, but also expression properties of the transgenes. This review summaries the two major patterns and the rules of T-DNA integration in Agrobacterim-mediated transformation, rules of T-DNA mediated by Agrobacterium tumefaciens, as well as research tools for flanking sequence amplification. It is attempted to provide references for researches on transformation and T-DNA integration mutation mediated by Agrobacterium tumefaciens.

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