Open AccessT-DNA integration patterns in transgenic plants mediated by <I>Agrobacterium tumefaciens</I>
Author(s) -
Lin Yang,
Feng Fu,
Wan-Chen Li
Publication year - 2011
Publication title -
yichuan
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.125
H-Index - 16
ISSN - 0253-9772
DOI - 10.3724/sp.j.1005.2011.01327
Subject(s) - agrobacterium tumefaciens , transformation (genetics) , biology , agrobacterium , transgene , genetics , transfer dna , genetically modified crops , dna , gene
The genetic transformation mediated by Agrobacterium tumefaciens has been widely applied to research of transgenic plants. As the vector of the exotic genes, the integration patterns of T-DNA fragments affects not only transformation efficiency and stability, but also expression properties of the transgenes. This review summaries the two major patterns and the rules of T-DNA integration in Agrobacterim-mediated transformation, rules of T-DNA mediated by Agrobacterium tumefaciens, as well as research tools for flanking sequence amplification. It is attempted to provide references for researches on transformation and T-DNA integration mutation mediated by Agrobacterium tumefaciens.