Open AccessAdvances of development of phage display systems
Author(s) -
Fanjing Meng,
Chaohui Zhang,
Yuncan Ai
Publication year - 2011
Publication title -
yichuan
Language(s) - English
Resource type - Journals
ISSN - 0253-9772
DOI - 10.3724/sp.j.1005.2011.01113
Subject(s) - phage display , computational biology , biology , selection (genetic algorithm) , genome , genetics , bacteriophage , synthetic biology , gene , computer science , escherichia coli , artificial intelligence , antibody
The phage display technology (PDT) was unique in genetic engineering and recombinant expression. The phage display systems (PDS) were platforms (kits) composed of genetic modified phages, helper phages, and host bacteria. This review concisely summarized the development of four types of PDS, based on M13, λ, T4, and T7 phages, in terms of phage molecular genetics and genetic (gene or genome) engineering. We addressed on the key components and their genetic (genomic) engineering for modifications, the technical features of different anchors, and the development progress and selection reference of those different kits.