Open AccessTSA improve transgenic porcine cloned embryo development and transgene expression
Author(s) -
Qingran Kong,
Jiang Zhu,
Bo Huang,
Huan Ye,
Wei Feng,
Yuhua Shi,
Zhong-Feng Liu,
Meiling Wu,
Liu Z
Publication year - 2011
Publication title -
yichuan
Language(s) - English
Resource type - Journals
ISSN - 0253-9772
DOI - 10.3724/sp.j.1005.2011.00749
Subject(s) - trichostatin a , reprogramming , transgene , biology , chromatin , embryo , microbiology and biotechnology , epigenetics , histone , cloning (programming) , transgenesis , histone deacetylase , somatic cell nuclear transfer , embryogenesis , blastocyst , genetics , cell , reproductive technology , dna , gene , computer science , programming language
Uncompleted epigenetic reprogramming is attributed to the low efficiency of producing transgenic cloned animals. Histone modification associated with epigenetics can directly influence the embryo development and transgene expression. Trichostatin A (TSA), as an inhibitor of histone deacetylase, can change the status of histone acetylation, improve somatic cell reprogramming, and enhance cloning efficiency. TSA prevents the chromatin structure from being condensed, so that transcription factor could binds to DNA sequence easily and enhance transgene expression. Our study established the optimal TSA treatment on porcine donor cells and cloned embryos, 250 nmol/L, 24 h and 40 nmol/L, 24 h, respectively. Furthermore, we found that both the cloned embryo and the donor cell treated by TSA resulted in the highest development efficiency. Meanwhile, TSA can improve transgene expression in donor cell and cloned embryo. In summary, TSA can significantly improve porcine reconstructed embryo development and transgene expression.