Open AccessExpression of cell cycle related proteins cyclin D1, p53 and p21<SUP>WAF1/Cip1</SUP> in esophageal squamous cell carcinoma
Author(s) -
DeChen Lin,
ZhiZhou Shi,
Liyan Xue,
Wei Chen,
Xin Xu,
Yaling Han,
Ning Lv,
Ming-Rong Wang
Publication year - 2010
Publication title -
yichuan
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.125
H-Index - 16
ISSN - 0253-9772
DOI - 10.3724/sp.j.1005.2010.00455
Subject(s) - cyclin d1 , cell cycle , cancer research , immunohistochemistry , cyclin , esophageal squamous cell carcinoma , tissue microarray , biology , cell , carcinoma , oncology , medicine , pathology , genetics
Dysregulation of cell cycle control, especially G1/S phase transition, is implicated in the pathogenesis of most human cancers, including esophageal squamous cell carcinoma (ESCC). However, the clinicopathological significance of aberrant expression of G1/S phase-related proteins in ESCC remains unclear. In the present study, cyclin D1, p21WAF1/Cip1 and p53 protein expression were examined in 148 ESCC cases using immunohistochemistry combined with tissue microarray. We then analyzed the correlations between their expression and clinicopathological parameters and found that overexpression of p53 was associated with lymph node metastasis (P=0.001). p21WAF1/Cip1 down-regulation was an independent prognostic factor by multivariate analysis (RR=0.418, P<0.001). Further more, array-CGH results revealed that cyclin D1 gene was amplified in 45.4% of ESCC patients. This study confirms that dysregulation of G1/S related genes is common in ESCC and reduced expression of p21WAF1/Cip1 protein can predict shorter overall survival time of patients with ESCC.